Vitamin C Determination By Indophenol Method

Vitamin C Determination By Indophenol Method. E2 vitc exp 1 Introduction Vitamin C (LAscorbic acid) is an important vitamin Lack of ascorbic acid in diet causes scurvy a disease characterized by weakness small hemorrhages throughout the body that causes gums and skin to bleed and loosening of the teeth Scurvy was a serious problem for English sailors in the 1600s and 1700s.

PDF file1 Accurately weigh out about 50 mg of the vitamin C powder and dissolve with distilled water in a 100 mL volumetric ask Stopper and shake the solution enough to dissolve all of the ascorbic acid Keep the vitamin C solution stoppered to avoid vitamin C oxidation by the oxygen in air.

Estimation of the ascorbic acid content of juices, purées

For the titration of ascorbic acid by the indophenol method I use to pipette directly between 1 to 10 mL of the fruit juice depending on the expected concentration (do a trial and error assay.

Determination of Vitamin C Chem Lab

Dissolve 0250 g vitamin C (ascorbic acid) in 100 ml distilled water Dilute to 250 ml with distilled water in a volumetric flask Label the flask as your vitamin C standard solution Standardizing Solutions Add 2500 ml of vitamin C standard solution to a 125 ml Erlenmeyer flask Add 10 drops of 1% starch solution.

2,6 Dichlorophenolindophenol an overview ScienceDirect

Add 5mL of working phenol solution mix fill to about 22mL then add 25mL of the working hypochlorite solution and rapidly mix Dilute to 25mL mix and store in the dark at 25ºC for 30 min to develop color Measure the absobance of the solution against a reagent blank at 630nm using 1cm cells Field Blanks.